Journal: Cancer reports (Hoboken, N.J.)

Article Title: Forced Vasohibin-1 Expression Increases Paclitaxel Sensitivity of Ovarian Cancer by Inhibiting Microtubule Activity.

doi: 10.1002/cnr2.70100

Figure Lengend Snippet: FIGURE 2 | Western blotting of cyclin B1 in VASH1-overexpressing or control luciferase gene-transfected ovarian cancer cell lines. (A) Cyclin B1 expression was weakly detected in control cells (SKOV-3/LUC and SHIN-3/LUC), whereas SKOV-3/VASH1 and SHIN-3/VASH1 cells clearly ex- pressed cyclin B1 at the position corresponding to a molecular weight of 55 kDa. β-actin in the cell lysate was used as a loading control. Each number indicates the relative protein expression level compared with β-actin as 1.0. (B) Densitometric analysis is shown in the column graph. Data are shown as the mean and SD. *p < 0.05 (vs. LUC gene transfected cells). LUC: luciferase, VASH: vasohibin.

Article Snippet: Antibody Catalog no. Company Anti- detyrosinated alpha tubulin rabbit polyclonal antibody ab48389 Abcam, Cambridge, UK Anti- alpha tubulin mouse monoclonal antibody sc- 32 293 Santa Cruz Biotechnology Inc., Dallas, TX, USA Anti- cyclin B1 rabbit monoclonal antibody 12 231 Cell Signaling Technology Inc., Danvers, MA, USA Anti- β- actin rabbit polyclonal antibody A2066 Sigma- Aldrich; Merck KGaA nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cnr2.70100 by loki revolution - C A PE S , W iley O nline L ibrary on [25/12/2024].

Techniques: Western Blot, Control, Luciferase, Transfection, Expressing, Molecular Weight

Journal: Scientific Reports

Article Title: Establishment and characterization of an orthotopic patient-derived Group 3 medulloblastoma model for preclinical drug evaluation

doi: 10.1038/srep46366

Figure Lengend Snippet: ( a ) MYC expression is demonstrated in primary tumour and xenografts with in situ hybridization. Scale bar is 20 μm. Western blotting detecting c-Myc ( b ) and cyclin B1 ( c ) in protein extracts isolated from MB-LU-181 cells JQ1 treatment. SK-N-AS neuroblastoma cells and human dermal fibroblasts (HDF) cells were included as positive and negative control for c-Myc expression, respectively. β-actin was used to ensure equal sample loading. ( d ) Cell viability measurement using WST-1 of ( d ) MB-LU-181 and DAOY cells treated with the indicated concentrations of JQ1 for 72-hours. IC 50 values between MB-LU-181 and DAOY cells were significantly different ( p < 0.0001 t-test; 0.27 μM in MB-LU-181 versus 10 μM in DAOY). Mean with SD from three independent experiments are displayed.

Article Snippet: After membrane blocking, the incubation with primary antibody was carried out with c-Myc (D84C12) Rabbit mAb (#5605, Cell Signaling, Danvers, USA) (dilution 1/1000), cyclin B1 Mouse mAb (sc-245, Santa Cruz Biotechnology, Santa Cruz, USA) (dilution 1/500), and β-Actin mouse mAb (#3700, Cell Signaling, Danvers, USA) (dilution 1/10,000), followed by incubation with a peroxidase-conjugated goat anti-rabbit antibody (#7074, Cell Signaling, Danvers, USA) (dilution 1/5,000) or horse anti-mouse (#7076, Cell Signaling, Danvers, USA) (dilution 1/4000 for cyclin B1 and 1/20,000 for β-Actin).

Techniques: Expressing, In Situ Hybridization, Western Blot, Isolation, Negative Control

Journal: eLife

Article Title: Systematic lncRNA mapping to genome-wide co-essential modules uncovers cancer dependency on uncharacterized lncRNAs

doi: 10.7554/eLife.77357

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-cyclin B1 (mouse monoclonal) , BD Pharmingen , #554177 , WB (1:1000).

Techniques: Transfection, Construct, Plasmid Preparation, Staining, Sequencing, SYBR Green Assay, Isolation, Proliferation Assay, Colony Assay, Cell Cycle Assay, Positive Control, Software